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In vitro fertilization (IVF) is an effective and relatively new treatment for patients with congenital obstructive azoospermia. We evaluated the in vitro fertilization of cryopreserved testicular tissue (TT) with purified spermatogonial stem cells (SSCs) from pre-pubertal cryptorchid patients. We also investigated the effects of time delay in transplantation of SSCs and the quality of testicular tissue in mouse IVF. Testicular tissue was obtained from patients with 2-4 years of age, who had undergone radical inguinal orchiectomy for cryptorchidism. SSCs were purified by magnetic cell sorting. Then, cryopreserved testicular tissue was thawed and transplanted into mouse seminiferous tubules under the kidney capsule. It was found that 3-4-fold higher reproductive efficiency was obtained in in vitro fertilization and 10-fold more total transplants were obtained in the SSC transplantation group compared to the TT transplantation group (pSave the Cheetahs Show

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