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Effect of exogenous adenosine on the distal colonic response to exogenous bradykinin in rabbits.
This study examines the effect of exogenous adenosine on the distal colonic response to bradykinin, a specific smooth muscle stimulant. The response was assessed by measuring changes in colonic tone, and accumulation of peptides in the venous effluent. In conscious, fasted, unanesthetized rabbits, bradykinin at 30 and 100 ng/kg given into the left femoral vein produced a 2-fold to 3-fold increase in colonic tone which was detected within 10-15 s after injection of bradykinin. The increase in colonic tone in response to bradykinin was unaffected by the presence of adenosine in the circulation at 30 or 100 ng/kg given intravenously. Bradykinin given into the systemic circulation also caused a dose-dependent increase in peptide accumulation in the venous effluent. When 10 micrograms/kg of bradykinin was infused into the left femoral vein, the peptide concentration in the left femoral vein increased by 362 +/- 51% (SE) of the basal peptide concentration, 1 min after infusion of bradykinin. Bradykinin produced a transient fall in systemic arterial blood pressure and a fall in mean arterial blood pressure in the left femoral artery, both of which were reversed when the bolus infusion was stopped. Adenosine administered at 30 and 100 ng/kg before 30 ng/kg of bradykinin produced no change in the peptide concentration in the left femoral vein. However, pretreatment with adenosine at 30 and 100 ng/kg before 100 ng/kg of bradykinin produced a striking and dose-dependent fall in the arterial peptide concentration, even when the basal peptide concentration was close to zero. The twofold increase in colonic tone in response to bradykinin was unaffected by pretreatment with adenosine, whereas the increase in colonic tone produced by 2.5 mM prostaglandin E
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