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Kabul Express 2006 Hindi Movie.The potential for laminin and collagen IV to protect human skin cells against exposure to ultraviolet (UV)-B radiation: depletion of laminin and collagen IV by enzyme digestions.
Collagen types IV and XVII, the main components of the basement membranes of the epidermis and the dermis, are known to be sensitive to UV-B-induced damage. Desmosome-associated laminin, on the other hand, is not attacked by trypsin, although pepsin and thermolysin digest it completely, probably because of its high content of tyrosine. We have examined the effects of UV-B radiation on laminin and collagen type IV, in order to gain an insight into the resistance of these two molecules against UV-B-induced damage. Laminin and collagen type IV are known to be important for the anchorage of keratinocytes to the basement membrane. The epidermis and the reticular dermis of human skin were exposed to UVA and UV-B radiation, and the effects of the radiation on the components were studied. Exposure to UVA and UV-B for 0.5-5 h resulted in an increased amount of laminin in the epidermal basement membrane, whereas no effects on dermal laminin were seen after 0.5 h of UVA radiation. Exposure to UV-B for 1 h resulted in total depletion of the laminin from the basement membrane, whilst trypsinization of the epidermis for 2 h did not cause such a depletion. The depletion of laminin by UV-B radiation seems to be due to an increased laminin breakdown into fragments, since no laminin fragments were found in the epidermal cells of irradiated skin. The dermis was only depleted of laminin by exposure to UVA for 24 h, even though this was associated with damage of collagen type IV. Since the depletion of laminin after UV-B radiation was found to be reversible and was mainly seen in the lower part of the basement membrane, it is possible that laminin may be important for protecting epidermal cells from UV-B-induced damage. UV-B radiation caused a slight depletion of type

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