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mosayile kuthira meenukal thatna kadavu kalayadaruIsolation and characterization of a novel human expressed gene, MDC1, and its widespread distribution of messenger RNA transcripts in the body.
A full-length cDNA, designated MDC1, encoding a protein of 1143 amino acid residues with a molecular mass of 130 kDa, was isolated from a human testis cDNA library. Its coding region was 98.6% homologous to that of LIS1, a human homolog of yeast Cdc13, which is localized at the centrosome and contributes to microtubule dynamics in yeast. The expression of MDC1 mRNA, however, was very limited. In contrast, its transcripts were widely distributed in various human tissues and the cells of 10 different human malignancies. As was shown by Northern blot analysis, MDC1 mRNA is highly expressed in small cell carcinoma of the lung and its overexpression can
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